QICS Data: Gene expression data of scallops Pecten maximus and mussels Mytilus edulis held in the vicinity of a sub-seabed CO2 release (2012)

The QICS project (Quantifying and Monitoring Potential Ecosystem Impacts of Geological Carbons Storage) was established to improve our understanding of the potential impacts of CO2 release on the environment and to develop tools and best practice for monitoring sub-seabed CCS reservoirs. To monitor the potential impact of a CO2 leak to surficial benthic megafauna, cages of bivalves (the common mussel Mytilus edulis Linnaeus, 1758 and the king scallop Pecten maximus (Linnaeus, 1758)) were deployed at the gas release site and at a reference site in the QICS experiment - both within Ardmucknish Bay, Oban, Scotland. Replicate individuals were sampled at six time points over a 125-day period, which spanned both the 37-day injection and recovery phases of the experiment, in order to establish impacts to molecular physiology. Samples of bivalves were also simultaneously sampled from a reference site within the bay in order to contrast changes in physiology induced by the gas release with naturally variability in the physiological performance of both species. There was no evidence of gene regulation of either selected carbonic anhydrases (CAx genes) or the alpha subunit of sodium potassium ATPAses (ATP1A genes) in individual bivalves collected from the CO2 gas release site, in either species. In the common mussel Mytilus edulis there was only evidence for changes with time in the expression of genes coding for different classes of carbonic anhydrase. It was concluded that the effects of the plume of elevated pCO2 on ion-regulatory gene transcription were negligible in both species. Pratt et al. 2015. No evidence for impacts to the molecular ecophysiology of ion or CO2 regulation in tissues of selected surface-dwelling bivalves in the vicinity of a sub-seabed CO2 release. International Journal of Greenhouse Gas Control. DOI:10.1016/j.ijggc.2014.10.001. QICS project website: www.bgs.ac.uk/qics/home.html.

QICS项目（地质碳封存潜在生态系统影响的量化与监测）旨在深化我们对二氧化碳排放对环境潜在影响的理解，并开发监测海底碳封存库的最佳实践工具。为监测二氧化碳泄漏对表层底栖大型无脊椎动物潜在影响，研究人员在气体释放现场及QICS实验中位于阿德穆克尼斯湾的参考站点（均在苏格兰奥班）部署了双壳贝类的笼子（包括常见的贻贝Mytilus edulis Linnaeus, 1758和扇贝Pecten maximus (Linnaeus, 1758)）。在125天的周期内，于六个时间点对重复个体进行采样，该周期涵盖了实验的37天注入和恢复阶段，以便确立分子生理学的影响。同时，从湾内的参考站点采集双壳贝类的样本，以对比气体释放引起的生理变化与两种物种生理性能的自然变异性。在从二氧化碳气体释放现场收集的所选碳酸酐酶（CAx基因）或钠钾ATP酶α亚基（ATP1A基因）的个体双壳贝类中，未发现基因调控的证据。在普通贻贝Mytilus edulis中，仅发现编码不同类别的碳酸酐酶的基因表达随时间变化的证据。因此得出结论，高碳酸二氧（pCO2）羽流的效应对两种物种的离子调节基因转录的影响可以忽略不计。Pratt等，2015年。在海底二氧化碳释放现场附近所选表面栖息的双壳贝类组织中，未发现对离子或二氧化碳调节分子生态生理学影响的证据。国际温室气体控制杂志。DOI：10.1016/j.ijggc.2014.10.001。QICS项目网站：www.bgs.ac.uk/qics/home.html。