Spatial Transcriptomics of Brains Infected with VEEV

Venezuelan equine encephalitis virus (VEEV) is a neurotropic alphavirus that causes neurological disease in both humans and equine. In this study we paired spatial transcriptomics and a mouse model of VEEV to generate a transcriptomic profile of infected or control brains at 6 days post infection. We identified regionally distinct myeloid and lymphocyte populations, contributing to our understanding of the neuroinflammation caused by VEEV. Spatial transcriptomics was performed on sections of brain prepared from either C3H/HeN mice infected intranasally with VEEV TC-83 at 6 days post-infection or healthy controls.