Effects of exogenous methionine on sulfur amino acids and glutathione levels in M. oryzae Δmet6 mutants and wild type.

Wild type M. oryzae isolate P1.2 was grown in liquid medium in absence (MM) or presence of 1 mM methionine (MM + Met) for 8 days. The Δmet6 mutants (M15.1, M22.1 and M23.1) were grown in liquid MM supplemented with 1 mM methionine (MM + Met) for 8 days. Cys (cysteine), CTT (cystathionine) and Hcys (homocysteine) were quantified by HLPC as described in Materials and Methods. Data were expressed in nmoles.mg-1 dry weight and represent the mean (± standard deviation) of two technical replicates derived from three biological replicates.**Fold change (Δmet6 / P1.2 MM + Met) was calculated using average value of the three mutant lines over wild type.°Differences are significant according to a t-test (5%).Effects of exogenous methionine on sulfur amino acids and glutathione levels in M. oryzae Δmet6 mutants and wild type.