Bench marking the assembly of viromes using Illumina, PacBio and Nanopore reads

Illumina PacBio ONT Flye Unicycler wtdbg2 (Redbean)
Sequencing of a mock community of bacteriophages with three different sequencing technologies , in triplicate.

Reads were assembled with multiple different assembly algorithms including 

Assembled contigs were compared to the known input phage genomes to calculate completeness of genomes 

All raw reads were deposisted in the ENA.

The attached file contains assembled contigs. 

Files are named in the following manner 

Library Number, Sequencing type , assembler used 

eg  lib1_PacBio_wtdbg2.fa
Library 1 sequenced using PacBio and assembed with wtdb2 

Data Update 

With advances in base calling the ONT data was re-basecalled.  

re-called ONT data is in  ONT_recalled.tar.gz and also avalible in the SRA .