Immune activation costs in Musca domestica driven by pirk knockout: altered resource acquisition and reallocation

RNA-Seq was employed for the dfferential expression analysis between wild-type(WT) and pirk-KO flies. The adult flies of dfferent strains were treated with a mixture of bacteria (E. coli and S. aureus) for 6h by injecton into the thorax. Three biological relicate samples were collected for each strain. The resuts demonstrated that Toll & Imd signaling pathway and central carbon metabolism pathway genes exhibited significant dfferences in expression levels between wild-type and mutant strains.