Improvement of hepatocellular carcinoma using hepatocyte-derived liver progenitor-like cells (HepLPCs): therapeutic, proliferation arrest, malignant biological behavior changes，signal pathway screening

We employed a co-culture experiment and found that human hepatocyte-derived liver progenitor-like cells (HepLPCs) significantly inhibited the proliferation of hepatocellular carcinoma (HCC) in a specific manner and altered their malignant biological behavior without affecting cell aging, autophagy, apoptosis and so on. To evaluate the mechanism of action, HepG2 and Hep3B after co-cultured with HepLPCs gene profiling were performed, and we observed, in addition to proliferative blockade induction, that cascade events originated from mitochondrial dysfunction. Global expression analysis of RNA sequencing data of expression levels of HepG2 and Hep3B in the presence or absence of hepatocytes derived liver progenitor-like cells (HepLPCs), and the global expression of HepG2 and Hep3B genes after combined inhibition of Notch1 and STAT3.