Budding yeast centromeric DNA and A+T rich bacterial DNA can function as centromeres in the fission yeast Schizosaccharomyces pombeÂ

We established a two-step approach to centromere-replacement (Figure 1A in outline, and Supplementary data in detail). FiC31 integrase was used to place a candidate sequence or an empty vector adjacent to the native centromere of chromosome 2 of CBS2777, and Bxb1 integrase was subsequently used to delete the native centromere.  Overall design: Examination of CENP-A binding on the S. pombe centromere 2 in 20 different strains