Sirt1 Deficiency Upregulates Glutathione Metabolism to Prevent Hepatocellular Carcinoma Initiation in Mice

Total RNA was extracted from Livers of 8 months 3 WT and 3 Sirt1 LKO mice, livers and tumors of DEN injected 3 WT and 3 Sirt1 LKO mice. mRNA was enriched using oligo(dT) beads. RNA-Seq was performed by Novogene Bioinformatics Technology Co., Ltd on Illumina HiSeq PE150.Sirt1flox5-6/flox5-6; Alb-cre mice (LKO) and Sirt1flox5-6/flox5-6 mice (WT) control cohorts were established by breeding Sirt1 flox 5-6/flox 5-6 females with Sirt1 flox5-6/flox5-6; Alb-cre males. To induce HCC, 14-day-old males were intraperitoneally injected with a single shot of 25 mg/kg DEN (Sigma N0756). All livers were collected during 9-11 a.m.