Stepwise activation of primary human memory CD4+ T cells

CD4+ T cells are a highly differentiated cell type that maintain enough transcriptomic plasticity to cycle between activated and memory statuses. We generated RNA-seq libraries from T-cells harvested from three independent blood donors under three separate conditions: unstimulated fresh ex vivo, IL-2 only stimulated and TCR+CD28+IL-2 stimulated, conferring increasingly stronger activation signals. . RNA-seq profiling of primary CD4+ memory T-cells, isolated from three blood donors and purified via FACS. Cells were treated with either IL-2, IL2+TCR or untreated. Donor 1 experiments were performed in triplicate, while donors 2 and 3 were performed in duplicate.