P_i uptake by parental, ΔTgPiT and ΔTgPiT::<i>PiT</i> parasites Intracellular parasites were cultivated for 24 h in P_i-depleted DMEM with 1% FBS prior to the phosphate uptake assay, which was performed as described in legend of Fig 1, in a P_i-depleted reaction medium at pH 7.4 containing 100 μM ³²P_i in the presence of 140 mM NaCl or choline chloride (ChCl) for energized and unenergized phosphate uptake conditions, respectively, at the indicated times.

Pi uptake by parental, ΔTgPiT and ΔTgPiT::PiT parasites Intracellular parasites were cultivated for 24 h in Pi-depleted DMEM with 1% FBS prior to the phosphate uptake assay, which was performed as described in legend of Fig 1, in a Pi-depleted reaction medium at pH 7.4 containing 100 μM 32Pi in the presence of 140 mM NaCl or choline chloride (ChCl) for energized and unenergized phosphate uptake conditions, respectively, at the indicated times.