Metadata record for the manuscript: Targeting nucleotide metabolism enhances the efficacy of anthracyclines and anti-metabolites in triple-negative breast cancer

Summary

This
metadata record provides details of the data supporting the claims of the
related manuscript: “Targeting nucleotide metabolism
enhances the efficacy of anthracyclines and anti-metabolites in triple-negative
breast cancer”.

The
related study sought to identify and characterise new therapeutic opportunities
to enhance current standard of care (SoC) chemotherapies that incorporate
anthracyclines and anti-metabolite 5-Fluorouracil (5-FU) in triple-negative
breast cancer (TNBC) through further modulation of pyrimidine and uracil nucleotide
metabolism pathways, hypothesising that this could be achieved through
inhibition of the gatekeeper enzyme, deoxyuridine 5’-triphosphate
nucleotidohydrolase (dUTPase), as this enzyme functions to prevent uracil
misincorporation into DNA.

Type of data:
cell survival, growth inhibition, DNA damage analysis, apoptosis, nucleotide
pool and in vivo data, Western Blot images

Subject of
data: MDA-MB-231 (human, ATCC HTB-26), MDA-MB-468
(human, ATCC HTB-132); Female Balb/c
mice; 4-6 weeks old; purchased from Envigo.

Sample size:
Groups of 5-6 mice for control and experimental conditions were used. Power
calculations were used to determine sample size.

Data
access

All the data
underlying figures 1-8 of the related article, including cell survival, growth
inhibition, DNA damage analysis, apoptosis, nucleotide pool and in vivo data, along
with data underlying supplementary figures 1, 2, 3, 4, 5, 7, 8 and Western Blot
images for supplementary figure 1a-b, are openly available as part of this figshare data record. Data files are
named in-line with the figure they underlie, and each may contain multiple tabs
for the sub-figures.

Corresponding author(s) for this study

Melissa
J LaBonte, Medicine, Dentistry and Biomedical Sciences: Patrick G Johnston
Centre for Cancer Research, Queen’s University Belfast, Belfast, BT9 7AE, UK. el:
[phone]; Email: [e-mail].

Study approval

The
mouse work was performed under an approved UK project license and approved by
the institutional Queen's University Belfast Animal Welfare Ethical Review Body
(AWERB).