BackgroundMost Yersinia pestis strains are known to express a capsule-like antigen, fraction 1 (F1). F1 is encoded by the caf1 gene located on the large 100-kb pFra plasmid, which is found in Y. pesti
We developed a high throughput platform that allows the unbiased, simultaneous discovery of antibodies and targets based on phenotypic binding profiles. The selection of antibody candidates is based o
a) The phage library, containing billions of different phages, was amplified from the bacterial stock. ScFv are fused to minor coat protein III and as such displayed on the phage surface. b) In a subt
* From sequencing screen of individual PC-binding clones after three rounds with PC-BSA conjugate and two rounds of phage display selection on whole AC. ** Compared to the VH, D and JH germline nucleo