Cell cycle analysis for Hep G2 apoptosis induced by ACE polysaccharides, ACE/CS and ACE/S for 24 h and 48 h.

Hep G2 cells were seeded at the density of 1.0 × 106 cells per 60-mm dish and treated with ACE polysaccharides (25 μg/mL), ACE/CS (ACE polysaccharides = 13.2 μg/mL) and ACE/S (ACE polysaccharides = 21.2 μg/mL). The nanoparticles without ACE polysaccharides (CSNP and SNP) were also examined. The cells without any treatment were defined as controls. Flow cytometry with excitation wavelength 488 nm/emission wavelength 630 nm was used for the analysis of PI-stained DNA, and data acquired from Cell Quest software based on a minimum of 105 cells per sample were analyzed using a one-way ANOVA followed by Duncan’s test. The results are shown as the mean ± standard deviation (S.D.; n = 3), which without a common superscript (a, b and c) at the same row were significantly different (pA. camphorata extracts, ACE/CS: ACE polysaccharides encapsulated by chitosan-silica nanoparticles, ACE/S: ACE polysaccharides encapsulated by silica nanoparticles, CSNP: chitosan-silica nanoparticles, SNP: silica nanoparticles.Cell cycle analysis for Hep G2 apoptosis induced by ACE polysaccharides, ACE/CS and ACE/S for 24 h and 48 h.