The studies of spliceosomal interactions are challenging due to their dynamic nature. Here we developed spliceosome iCLIP, which immunoprecipitates SmB along with snRNPs and auxiliary RNA binding prot
Knockdown and overexpression of C* proteins in Hela and HEK293 cells was investigated by RNA-Seq. Overall design: HeLa cells were transfected either with the indicated siRNAs and analyzed by RNA-Seq.
Precise 5' splice site recognition is essential for both constitutive and regulated pre-mRNA splicing. The U1 snRNP specific protein U1C is involved in this first step of spliceosome assembly and impo
Alternative precursor-mRNA splicing (AS) is a key regulation of gene expression and protein diversity. AS is highly happened in the nervous system and misregulation of AS caused neurological diseases.