Detection of CYP2C19*2 allele among Helicopacter pylori-infected patients in two tertiary hospitals in Khartoum-Sudan

Antral biopsies were collected from 30 patients attending endoscopy units. EXtraction of DNA was performed through QIAampR DNA mini kit. Samples were screened for Urease C (UreC) gene of H.pylori using conventional PCR, Detection of CYP2C19*allele was performed in positive H.pylori samples using Real time-PCR. Ethical clearance was obtained from the scientific committee of the Virology department of central laboratories- Ministry of higher education and Research. Consent was also taken from hospital managers and patients after explanation of research purpose and procedures.