Friend or Foe? Primary Tumor represses distant metastases' growth via exosomal miRNA-1

Recently, exosome has been treated as a key mechanism for cell-to-cell communication. We thus demonstrated the roles of exosomes for the encoding messages between primary tumors and metastases. To investigate the difference between pTDE miRNA (primary tumor-derived exosomal miRNA) and mDE miRNA (metastases-derived exosomal miRNA), we isolated exosomal miRNA from each cell lines. Next, we performed small RNA sequencing for miRNA profiling. Compared with pTDE and mDE miRNAs, miR-1 is specifically abundunt in pTDE and also shows therapeutic effects to repress the growth of metastases after primary tumor resection. Profiling both pTDEs and mDE miRNAs, we isolated exosomal miRNAs from CHMp (primary tumor) and CHMm (metastases) with two replicates.