H&E, TTF1, immunofluorescence staining and Digital Spatial Profiling of histologically heterogeneous lung adenocarcinoma FFPE sections.

Please refer to the methods section of the paper for more detailed explanations. In summary: <strong>chuv_imaging_dataset_annotation.xlsx</strong>: table containing imaging assays available for each sample and histologic patterns present. <strong>he_images.zip</strong>: high-resolution images of FFPE sections stained with H&amp;E and acquired at 20x magnification. <strong>ttf1_images.zip</strong>: high-resolution images of FFPE sections stained with TTF1 and acquired at 20x magnification. <strong>if_images.zip</strong>: high-resolution images of FFPE sections assayed with multicolor immunofluorescence (markers: CD20, CD4, CD8, CD68, Ki67) and acquired at 20x magnification. <strong>DSP_data_and_annotations.xlsx</strong>: tables containing Digital Spatial Profiling (DSP) data (in SNR) and annotations for batch1 and batch2. <strong>all_snapshots.zip</strong>: low-resolution snapshots of all images uploaded in this repository (H&amp;E, TTF1, immunofluorescence - with all 5 colors together and with each color separately), with histologic pattern boundaries drawn on the images; immunofluorescence snapshots of DSP ROIs (batch1) and their position on each slide. Color code for histologic pattern boundaries: grey = normal, blue = lepidic, sea green = papillary, orange = acinar, red = solid.<br> Color code for immunofluorescence channels: cyan = CD20, blue = CD4, yellow = CD8, red = CD68, green = Ki67.<br> Color code for DSP snapshots: sea green = CD3, red = CD45, yellow = CD20, blue = DAPI