Differential microRNA expression along with progression of diet-induced atherosclerotic plaque using Microarray

To understand the changes in microRNA expression regulating specific genes at different stages of diet-induced atherosclerotic development, double knock out Apobtm2Sgy/Ldltm1Her mice were fed with either high-fat or normal chow diet. The microarray was performed using total RNA extracted from ascending aorta of the animals. Double knockout mice fed with rich cholesterol diet and a normal diet were sacrificed after 0, 6, 1, 8 and 30 weeks and RNA was extracted for Microarray analysis