NMJ fragmentation in DLS/LeJ mice.

Gastrocnemius muscles of healthy and dilute mice were explanted at P7, P14 or P20. Muscles were fixed, sliced and stained with BGT-AF647. Upon confocal imaging and image processing, the numbers of NMJ fragments identified as discontinuous gutters of each NMJ were counted. Data, mean±s.e.m. (n>100 analysed NMJs per condition from at least two different animals).