Illumina Bead expression array data from Human B cells treated with miR-29 over expression

Human Tonsillar B cells were isolated and transfected with miR-29 mimic molecules or transfection controls. B cells were subsequently cultured in class switching media for 24 hours and subjected to transcriptome analysis using the Illumina HumanHT-12 v4 Expression BeadChip array platform. B cells derived from 3 donors are submitted. Total B cells were isolated from discarded tonsillar tissue from 3 donors following routine tonsillectomy (following informed consent) and transfected (nucleofection) with miR-29 mimic molecules or transfection controls prior to cultured in the presence of IL-4 and anti-CD40 stimulation. After 24 hours culture total RNA extracted. RNA was then reverse transcribed, amplified and biotin labelled prior to profiling on Illumina Human HT-12 v4 expression arrays.