Culture condition comparisons of WTC11 hiPSC by single-cell RNAseq and featuring differences between cE8, hE8 and B8+ media

This single-cell RNA-sequencing experiment was performed for the study “Refined and benchmarked homemade media for cost-effective, weekend-free human pluripotent stem cell culture.” Human induced pluripotent stem cells (hiPSCs) from the WTC-11 line were adapted to three defined media: cE8, hE8, and B8+. Two adaptation rounds for each medium were analyzed. The goal of this experiment is to assess the heterogeneity of hiPSCs cultured in three media and whether the weekend-free culture schedule affects this heterogeneity.