Sensitive fluorescence detection of p-syn and t-syn over time.

Shown in A are fluorescence images of nuclei (Blue), t-syn (green), and p-syn (red) with the exposure time of 40 ms, 200 ms, and 5000 ms, respectively. Cells were imaged (top row) and subsequently removed from tetracycline to induce α-synuclein expression. Cells were then reimaged at day 4 (middle row) and day 9 (bottom row). Shown in B, image intensity increased following induction of the synuclein transgene, consistent with increased p-syn and t-syn expression over time.