ChIP-seq Analysis for H3K4me3 in Neomycin-induced Lgr5+ Progenitor Cells of Neonatal Mouse Cochlea

In the study, we detailedly manifested the regulatory roles of histone modifications in neomycin-induced proliferation of Lgr5+ progenitor and HC regeneration by integrated RNA-seq and ChIP-seq analysis. we found 94 differentially expressed genes which are related to differential binding regions via analyzing ChIP-seq and RNA-seq data. we are delighted to find that H3K4me3 can modulate the expressions of 12 genes to involve in cell cycle, neural development, inner ear development, Wnt and Hippo signaling pathways to regulate proliferation and HC regeneration based on the protein interaction network and gene ontology (GO) functional annotation analysis. Examination of H3K4me3 modification in the mouse cochlea Lgr5+ progenitor cells under neomycin-treated and untreated conditions.