Dataset proteomics: Analysis of change in protein expression in Saccharomyces cerevisiae upon shift from chemostat to feast/famine regime for different sugar substrates

This proteomics dataset was generated to analyse the changes in protein expression between a chemostat and feast/famine regime culture of <em>Saccharomyces cerevisiae</em> CEN.PK113-7D grown on sucrose, maltose and fructose at D = 0.1 h^-1. Proteome composition of the taken samples was subsequently measured by DSM, and the obtained dataset was analysed for significant changes in protein expression between chemostat and feast/famine conditions. <br> Files in this item:<br> 1) Materials and Methods:<br> Materials_methods_proteomics_sugar_substrates_chemostat_FF_shift_Verhagen.docx<br> <br> Describes the methodology of the reactor experiment, sampling and proteomics data analysis.<br> <br> 2) Proteomics data:<br> Data_proteomics_sugar_substrates_shift_chemostat_FF_Verhagen.xlsx<br> <br> Contains raw data received from DSM's proteomics measurements facility and subsequent statistical analysis.<br> Read sheet 'Description' for explanation of other available sheets in the file

本蛋白质组学数据集旨在分析以蔗糖、麦芽糖和果糖为碳源，在稀释率D=0.1 h⁻¹条件下培养的酿酒酵母（Saccharomyces cerevisiae）CEN.PK113-7D在恒化器（chemostat）与饱食-饥饿（feast/famine）培养模式下的蛋白质表达差异。后续通过DSM完成了采集样本的蛋白质组组成测定，并针对所获数据集分析了恒化器与饱食-饥饿培养条件下蛋白质表达的显著变化。
本数据集包含以下文件：
1) 材料与方法：Materials_methods_proteomics_sugar_substrates_chemostat_FF_shift_Verhagen.docx
该文档详细阐述了反应器实验、样本采集及蛋白质组学数据分析的实验方法。

2) 蛋白质组学数据：Data_proteomics_sugar_substrates_shift_chemostat_FF_Verhagen.xlsx
该文件包含从DSM蛋白质组学测定平台获取的原始数据及后续统计分析结果。
请查看文件内的"说明（Description）"工作表，以了解文件中其他可用工作表的相关说明