Differential gene expression analysis on cells expressing Wild type GFP-MAB21L1 and its mutant form GFP-Arg51Leu and GFP-Arg51Gln

RNA Sequencing analysis was performed on RNA isolated from cells tagged with GFP-MAB21L1 and its mutant form GFP-Arg51Leu and GFP-Arg51Gln with and without treatment of tetracycline for 12 hrs. Transcript-level quantitation was performed using Salmon (v0.8.2) against the GRCh38 Ensembl reference transcriptome (release-89).Transcript-level counts were summarized to gene level using the bioconductor package tximport (v1.4.0). Differential expression analysis was performed with the bioconductor package DESeq2 (v1.30.0) using the Wald significance tests. 12 samples , with 6 groups and 2 replicates per group.