Codes and data used for the Manuscript titled "<b>Diet-induced miRNAs regulate adult neurogenesis and functional activity of nascent neurons in the hypothalamus</b>"

Neurogenesis in the hypothalamus upon high fat diet (HFD) feeding regulates the feeding circuit. HFD induces the neurogenesis of β2 tanycytes in young- adult mice. However, molecular mechanisms of tanycytic neurogenesis and their functional integration into the feeding circuitry is poorly understood. We investigated the role of miRNAs in the regulation of HFD-induced tanycytic neurogenesis. miRNA arrays identified a cohort of HFD-induced, differentially-regulated miRNAs in BrdU⁺ β2-tanycytes. These miRNAs arise from different chromosomes, rather than a single cluster. <i>In silico</i> network analysis on the predicted targets of all five HFD-induced miRNAs and reporter assays identified a subset of targets that influence neurogenesis and neuronal differentiation. HFD-induced miRNAs drive a molecular program leading to the functional integration of nascent neurons; introduction of a miRNA sponge sequestering all five miRNAs abolishes it. Diet-regulated newborn neurons preferentially differentiate into AgRP+ neurons that functionally integrate into the feeding circuit.