Splenocytes, peripheral blood cells, peritoneal cells and tumor cells were washed with PBS and were counted. Cells were centrifuged at 400 g for 5 min and supernatant was removed. Cells pellet (< 3 x
RNA was sequenced from Normal and DP 16 mice to identify consistent changes in gene expression across cohorts Overall design: Cells were cultured at subconfluency and RNA harvested for sequencing
