Proteins raw data using CT-1 in MDA-MB-231
收藏国家人口健康科学数据中心2026-06-01 收录
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https://www.ncmi.cn/phda/dataDetails.do?id=CSTR:17970.11.A001G.202410.367.V1.0
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资源简介：
The MDA-MB-231 cells were treated with CT-1 for 24 h. Then the cells were collected for proteomic analysis. SDT buffer was used for sample lysis and protein extraction. Protein digestion by trypsin was performed according to filter-aided sample preparation (FASP) procedure described by Matthias Mann. Then 100 μg peptide mixture of each sample was labeled using TMT reagent according to the manufacturer’s instructions. LC-MS/MS analysis was performed on a Q Exactive mass spectrometer that was coupled to Easy nLC for 60/90 min. The MS raw data for each sample were searched using the MASCOT engine embedded into Proteome Discoverer 1.4 software for identification and quantitation analysis.
提供机构：
北京蛋白质组研究中心
创建时间：
2024-10-30
