RNA seq on tumors from two groups of mice myofibroblast-collagen1 knockout group and control group)

RNA sequencing analysis on tumor samples from two groups of mice (1) KPPF;Col1smaKO myfibroblast-collagen1 knockout group and (2) KPPF control group. Collagen1 is the most abundant extracellular matrix protein component in desmoplastic pancreatic tumors. In this study, we establish the transgenic mouse model KPPF;Col1smaKO mice with pancreatic tumors deleted for myofibroblast-derived collagen1. Then we use RNA-seq to examine the global changes of gene expression profile in tumors of KPPF;Col1smaKO mice (as compared to control KPPF mice). Overall design: A total of 7 RNA samples from tumors from two groups of age-matched mice (1) KPPF;Col1smaKO myfibroblast-collagen1 knockout group, 4 mice; and (2) KPPF control group, 3 mice.