Whole-brain recordings of adult Drosophila using light field microscopy along with corresponding behavior or stimuli data.

This dataset contains whole brain calcium or voltage recordings of adult Drosophila. They were recorded with light field microscopy, which permits ultra-fast whole-brain acquisition (up to 200Hz) albeit at lower spatial resolution than other techniques such as confocal microscopy. In some of the calcium data, the fly was behaving (mostly walking or grooming) and the behavior data is included. Other voltage and calcium datasets present response to stimuli (puffs of odor or flashes of UV light).

One recording typically lasted one or two minutes. There are around 5 flies per condition. The whole brain fluorescence recordings are in NifTI format, and can be opened in ImageJ using the NifTI Input/Output plugin, with matlab using MRIread, or with python using nibabel (see scripts provided). Examples of alignment to an anatomical template, as well as results from source extraction using PCA/ICA are also provided.

See also a github repository: https://github.com/sophie63/FlyLFM, as well as a publication based on this dataset:
Fast near-whole brain imaging in adult Drosophila during responses to stimuli and behavior. Sophie Aimon, Takeo Katsuki, Tongqiu Jia, Logan Grosenick, Michael Broxton, Karl Deisseroth, Terrence J. Sejnowski, Ralph J Greenspan bioRxiv 033803; doi: https://doi.org/10.1101/033803