five

Global accessibility of mitotic chromosomes

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NIAID Data Ecosystem2026-03-11 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE85184
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To test if mitotic chromosomes have decreased accessibility, we utilized Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) analysis where the integration of sequencing-compatible adaptors by the Tn5 transposase is directly correlated with the accessibility of genomic regions We performed ATAC-seq in 50,000 asynchronous cells as well as 50,000 Nocodazole-synchronized mitotic cells in two biological replicates each.
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2019-05-15
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