E11bio PRISM

This dataset was generated using E11.bio's PRISM technology (Protein Reconstruction and Identification through Multiplexing), a platform that combines viral barcoding, expansion microscopy, and iterative immunolabeling for large-scale neuronal reconstruction.Neurons in the mouse hippocampal CA3 were transduced with a library of adeno-associated viruses (AAVs) encoding diverse “protein bits”—small epitope tags that act as combinatorial barcodes. Tissue was then processed with an expansion microscopy protocol, physically enlarging the sample ~5× to achieve an effective voxel size of ~35 × 3...本数据集通过E11.bio公司的PRISM技术（Protein Reconstruction and Identification through Multiplexing，多重复用蛋白质重建与识别技术）生成，该平台整合了病毒条形码技术、扩展显微镜技术与迭代免疫标记技术，可用于大规模神经元重建。研究人员将编码多样化“蛋白质片段”的腺相关病毒（AAV）文库转导至小鼠海马CA3区的神经元中，此类“蛋白质片段”为可作为组合条形码的小型表位标签。随后，研究人员采用扩展显微镜实验流程对组织样本进行处理，将样本物理放大约5倍，以实现约35×3……的有效体素尺寸。