Freshwater Prokaryotes from Ponds and Brooks Targeted Locus (Loci)

Despite their global distribution and ecological importance, little information about prokaryotic diversity is available from small, shallow and ephemeral freshwater systems. The goal of this project is to monitor monthly and compare prokaryotic diversity in one brook and four shallow ponds located in the "Natural Regional Park of the Chevreuse Valley", France during 2 years (April 2011-April 2013). The study is based on the identification and comparison and time tracking of operational taxonomic units (OTUs) infered from massive pyrosequencing of amplified 16S rRNA gene fragments of prokaryotes in the 0.2-5 µm-size range.