HDAC inhibitor TSA and HAT inhibitor Garcinol neither affect gustatory responsiveness nor non-associative learning.

Gustatory responsiveness, sensitization, or habituation of honeybees was tested 2 h after injection of TSA, or Garcinol, or the corresponding vehicle.The data for responsiveness and sensitization show the percentage of animals that elicited the proboscis (PER). The habituation criterion presents the mean ± SD of responses until the bees show five successive failures to elicit a PER after repetitive sucrose stimulation to an antenna. The number (n) of tested bees is indicated in brackets. As revealed by Student's t-test (habituation) and Chi-square/Fisher exact test (responsiveness and sensitization) TSA and Garcinol both have no effect on the tested behavioral parameters (ns; p>0.6).