Bactericidal activity of selected inhA inhibitors - raw data

The aim of the research was to identify new inhibitors of mycobacterial cell wall biosynthesis that inactivate the FAS2 system through interaction with enoyl acyl carrier protein reductase (InhA).Raw data of biological part have been deposited including: (i) Assessment of minimum inhibitory concentration (MIC) for the growth of tuberculosis bacilli and Mycobacterium abscessus obtained using the MABA test (microplate alamar blue assay). Initial screening of antibacterial activity was conducted at a concentration of 125 μg/mL, and then MIC values were determined for compounds showing activity (numerical data in Excel, JPEG images of plates); (ii) Determination of bactericidal concentration of selected compounds against M. tuberculosis performed using colony forming unit (CFU) count assessment, comparing the number of viable bacterial cells before compound addition, after 168 and 336 hours of incubation in the presence of test compounds. Data are included in an Excel table. (iii) Assessment of cytotoxicity of test compounds against murine fibroblast cell line L929. Data are included in Excel format. (iv) Evaluation of the effectiveness of test compounds against tuberculosis bacilli forming biofilms. Raw data obtained and statistical significance analyses (Ordinary One-way ANOVA) are included in an Excel file. (v) Assessment of the activity of test compounds against tuberculosis bacilli located within human macrophages, human monocyte-derived macrophages (MDMs), was performed after 48-hour incubation in the presence of test compounds by plating on solid media (CFU). The obtained data and their statistical analysis (Ordinary One-way ANOVA) are presented in an Excel file.