Fractionation of phosphopeptides using reversed phase microcolumn prior to LC-MS analysis
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资源简介：
A protocol for an improved phosphopeptide identification in tryptically digested complex peptide samples is described. The common TiO2 based phoshopeptide enrichment is coupled to a simple peptide fractionation protocol with following LC-MS analysis of the obtained fractions and proteomic identification of phosphorylated peptides. The fractions are obtained by an optimized protocol for peptide fractionation using a home-made capillary column coupled to a microgradient elution using a gastight microsyringe. The protocol leads to a substantially increased number of phosphopeptides (more than twice).
创建时间：
2020-08-31
