Complete genome sequence of Sphingomonas sp. gentR, a high-level gentamicin-resistant bacterium

We present the complete genome sequence of Sphingomonas sp. gentR, a strain exhibiting high-level resistance to gentamicin (MIC = 40 mg/mL). The genome was assembled from hybrid Illumina and Nanopore sequencing data into a gap-free sequence of 4.0 Mbp, comprising one chromosome and two plasmids. A total of 3,692 coding sequences were predicted, with comprehensive functional annotation revealing genes associated with antibiotic resistance, stress adaptation, and metabolic diversity. Three confirmed resistance genes—ANT(2″)-Ia, ANT(3″)-IIa, and Sul1—were co-localized within a genomic island on plasmid B. This dataset provides insight into the genetic basis of high-level aminoglycoside resistance in Sphingomonas and serves as a valuable resource for studying horizontal gene transfer, environmental adaptation, and bioremediation potential.