Transcriptome-wide view of endosomal mRNP transport [iCLIP ]

Spatiotemporal protein expression is mediated by active transport and local translation of mRNAs. Key factors of the transport machinery are RNA-binding proteins (RBPs) that recognise mRNAs as cargo for motor-based transport. However, a global view of transported mRNAs with cognate RBP binding sites is missing. Here, we cast a transcriptome-wide view on endosomal mRNP transport in Ustilago maydis by studying the newly identified endosomal RBP Grp1 and the key transport RBP Rrm4 in a comparative iCLIP approach. This uncovered thousand of cargo mRNAs bound by both RBPs. Overall design: in vivo iCLIP data to study the RNA binding of components of the mRNA transport machinery in hyphae of the phytopathogenic fungus Ustilago maydis