Microarray based gene expression profiling of RNA binding protein, RBM10 in HEK 293 cells

To determine the target genes of RBM10,we have employed microarray based gene expression profiling by knocking down RBM10 in HEK 293 cells. Microarray analysis after RBM10 knockdown on HEK 293 cells showed that over 1000 genes were down regulated while another 800 genes up regulated as a result of the knockdown. Among the down regulated genes, we found the significant presence of cardiovascular disease related genes, especially cardiac hypertrophy and heart failure. HEK 293 cells was transfected with siRNA oligos (both control siRNA and RBM10 specific siRNA) using Oligofectamine reagent and cells were harvested 48-hours post-transfection. Three independent experiments were conducted for both control siRNA and RBM10 siRNA treatment.