Plumes and Blooms: Microbial eukaryote diversity and composition

These are amplicon sequencing data collected during Plumes and
Blooms (PnB) cruises conducted from March, 2011, through
September, 2014. The V9 hypervariable region of the 18S rRNA gene
derived from microbial eukaryotic communities was amplified and
sequenced from 345 discrete seawater samples. Sample collection
and laboratory methods are described in Catlett et al. 2020 and
Catlett et al. in review. Bioinformatic and data manipulation
methods follow those employed in Catlett et al. in review. The
data are provided in two tables: one includes amplicon sequence
variant (ASV) sequences and relative sequence abundances for each
sampling event, and the other includes ASV taxonomy predictions
for each ASV sequence.

References:

Catlett, D., P. G. Matson, C. A. Carlson, E. G. Wilbanks, D. A.
Siegel, and M. D. Iglesias‐Rodriguez. 2020. Evaluation of accuracy
and precision in an amplicon sequencing workflow for marine
protist communities. Limnol. Oceanogr.: Methods. 18(1): 20-40.
https://doi.org/10.1002/lom3.10343.

Catlett, D., D. A. Siegel, P. G. Matson, E. K. Wear, C. A.
Carlson, T. S. Lankiewicz, and M. D. Iglesias‐Rodriguez. In
review. Integrating phytoplankton pigment and DNA meta-barcoding
observations to determine phytoplankton community composition in
the coastal ocean. Limnol. Oceanogr.