Spatially resolved transcriptional landscape of human knee cartilage in patients with osteoarthritis (OA) and non-OA controls

The characterization of knee articular cartilage changes with different zones -- articular surface, (AS), superficial zone (SZ), middle zone (MZ), and deep zone (DZ) -- based on the organization of the tissue and the alignment degree of collagen fibers. To comprehensively explore the spatial landscape of chondrocytes on human knee articular cartilage, we carried out the laser capture microdissection (LCM) coupled with full-length mRNA sequencing. Overall design: 124 samples of 9 cartilage samples from 4 patients with OA and 1 non-OA control