Transient epigenetic reprogramming during adult zebrafish muscle regeneration

Genomic reprogramming and cellular dedifferentiation are critical to the success of de novo tissue regeneration in lower vertebrates such as zebrafish and axolotl. ChIP-seq of the histone modifications H3K27Ac, H3K27me3, and H3K4me3 was used to characterize early epigenetic changes in a zebrafish in vivo model of adult muscle regeneration. Overall design: Histone H3 modification in Danio rerio lateral rectus muscle before and after injury. This study consists of 9 ChIP-seq samples and 1 input sample. Chromatin from control, 9 hours post-injury (hpi), and 18 hpi tissue from multiple wild-type specimens was assayed by ChIP-seq for H3K27Ac, H3K27me3, and H3K4me3 modification.