Changes in physicochemical properties.

P-values from paired Wilcoxon tests of differences in amino acid composition between the mesophilic and psychrophilic groups. Complete sequences: The results for all the 66 membrane protein cluster. Results of 52 transmembrane proteins (TMP) are shown in columns 3–5. Tc: complete sequence of all TMP cluster. Tm: membrane helices of TMPs. To: sequence parts of TMPs outside membrane. Results from all 11 signal peptide clusters are shown in column 6–8. Sc: complete sequence of all signal peptides. Sm: signal sequence of signal peptides (anchor). So: sequence parts outside membrane. Differences with p-values< are shown in bold. Arrows indicate direction of change from the mesophilic to the psychrophilic group and are based on the median value of the observed changes.