RNA-seq of ISL1 overexpression during islet differentiation.

This study aims to investigate the impact of ISL1 overexpression on hPSC-derived islet differentiation. hPSCs from four genotypes (WT, ISL1-/-, PDX1-/-, and PAX6-/-) were differentiated to pancreatic progenitors (day 11), transduced with ISL1-IRES2-BFP or BFP control lentivirus, and further differentiated to SC-islet (day 18). The BFP+ cells were isolated by flow cytometry sorting and subjected to RNA-seq to assess transcriptional responses to ISL1 overexpression across different genetic backgrounds. Overall design: This study generated RNA-seq on 21 samples. Cells were transduced with either ISL1-IRES2-BFP or BFP control on day 11 of islet differentiation and harvested on day 18. Most conditions were represented by three biological replicates. Three conditions, ISL1 overexpression in WT, BFP control overexpression in WT, and ISL1 overexpression in PDX1-/-, were represented by two biological replicates.