Tn-seq wig files for wild-type and ΔrecG library replicates

Maintaining the integrity of the genome is of utmost importance for cell division and propagation. In Escherichia coli, the RecG protein has been implicated in processing branched recombination intermediates during DNA repair processes, but the primary cellular role(s) of RecG and the repair pathways in which it acts have been difficult to define. To gain additional insight into RecG function, we employed transposon sequencing (Tn-seq) to identify recG genetic interactions and reveal complementary or redundant functions. The strongest hits from the screen were the dam, uvrD, rnhA, radA, and rep genes. The conditional importance of these five genes in cells lacking recG was confirmed using a plasmid-based assay, revealing synthetic lethal interactions for most double deletion strains. Several of the synthetic lethal gene combinations (with uvrD, rep, and radA; but not rnhA or dam) were suppressed by deletion of recF or recO, indicating that their genetic relationships involved roles in p..., , Can use MochiView of IGV to view gene insertion profiles., # Tn-seq data wig files for wild-type and *recG E. coli* K12 MG1655 library replicate samples

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Tn-seq libraries were created in triplicate for wild-type MG1655 *E. coli* and *E. coli* lacking the *recG* gene. Libraries were sequenced using Illumina NGS technology and raw sequencing data was analyzed to map transposon insertions to genes in each library replicate.

## Description of the data and file structure

These files provide the number of insertion sequencing reads present at each chromosomal location from each dataset. The files can be viewed as transposon insertion profiles for genes or intergenic regions in programs such as MochiView and IGV.

## Sharing/Access information

The data shared here is part of a manuscript that has been submitted to the Journal of Bacteriology. Raw sequencing data can be found here: