Calponin-Homology Domain mediated bending of membrane associated actin filaments

This dataset contains data from experiments described in 'Palani S, Ghosh S, Ivorra-Molla E, Clarke S, Suchenko A, Balasubramanian MK, Köster DV. 2021. Calponin-homology domain mediated bending of membrane associated actin filaments. Elife 10. doi:10.7554/eLife.61078'. This dataset consists of fluorescence microscopy images obtained by total internal reflection fluorescence (TIRF) microscopy. Using an in vitro approach, we studied the effect of the IQGAP protein fragment Rng2(1-189) on the geometry of actin filaments when tethered to supported lipid bilayers all reconstituted from purified proteins. The main findings are that Rng2(1-189) bends actin filaments into tight rings when tethered to supported lipid bilayers.