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Oligonucleotide primers used for cDNA cloning or RT-PCR analysis.

NIAID Data Ecosystem2026-03-07 收录

数据链接：

https://figshare.com/articles/dataset/_Oligonucleotide_primers_used_for_cDNA_cloning_or_RT_PCR_analysis_/568386 数据链接链接失效反馈

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资源简介：

*Position: The nucleotide position in each accession number.

应用场景：

创建时间：

2013-02-21

病毒基因工程

RT-PCR

RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted

DataCite Commons2023-01-19 更新80

Fig6G-6I_PCR_MDCK_A549_R3_LG403-404_annotated.tif

病毒基因工程

RT-PCR

RT-PCR analysis of viral RNA to verify presence of inserted sequences. A 51 bp segment of the STOML2 gene was inserted in the M segment of A/Puerto Rico/8/1984 (H1N1) ("PR8"). Viral RNA was extracted

NIAID Data Ecosystem50

Primers for specific amplification of Seg-2 of BTV-6, by RT-PCR.

蓝舌病毒分子分型

RT-PCR

*Individual primers are identified by the BTV serotype (e.g. BTV-6) followed by the number 2 (to indicate Seg-2), then a number to indicate the relative amino acid position of the primer within VP2, f

NIAID Data Ecosystem50

Sequences of primer pairs and probes used for real-time RT-PCR.

分子生物学

RT-PCR

Sequences of primer pairs and probes used for real-time RT-PCR.

NIAID Data Ecosystem60

Additional file 3: Table S2. of Viral metagenomics of aphids present in bean and maize plots on mixed-use farms in Kenya reveals the presence of three dicistroviruses including a novel Big Sioux River virus-like dicistrovirus

病毒宏基因组

RT-PCR

List of primers used for RT-PCR and sequences generated. (XLSX 18 kb)

DataCite Commons2024-12-18 更新50