qPCR parameters providing the standard curve for each primer pair on 12 reference genes.
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Relationship between Cq values and RNA concentration was calculated by linear regression to find a slope and intercept that predicts cDNA amounts and correlation coefficient (R2). QPCR efficiencies (E) were calculated based on the standard curve according to the equation [E = 10(−1/slope)−1]×100 and are expressed as a percentage.
创建时间:
2013-03-15



