The role of BAF60C in type 2 diabetes——RNAseq

This research identify reduced expression of BAF60C in diabetic islets and demonstrate that altered ß-cell BAF60C levels strongly influence metabolic homeostasis. Genetic loss of BAF60C in ß-cells exacerbates hyperglycemia and islet dysfunction under metabolic stress, whereas ß-cell–specific restoration of BAF60C produces protective effects. These findings establish BAF60C as an important regulator of ß-cell integrity and disease progression in T2D. Overall design: We used RNA-Seq to assess the effects of different treatment in mouse islets, containing RNA-Seq from islets isolated from beta-cell-specific ablation (BcBKO) mice, REG3B treated mice, WT mice, which elaborates the changes in transcriptional networks. Islet RNA-Seq data from flox/flox and BcBKO Pdx1-cre mice were fed with chow diet or HFD for 3M. You can find detailed information on sample collection and data analysis pipeline in our manuscript.