Expression data from human foreskin fibroblasts (HFFs) infected with Toxoplasma gondii

Through targeted CRISPR screening of the Toxoplasma gondii secretome, we identified GRA57 as a novel secreted effector required for survival in interferon-gamma (IFNg)-activated human foreskin fibroblasts (HFFs). In this experiment we aimed to determine if GRA57 affects the host cell transcriptional response to Toxoplasma, and also whether deletion of GRA57 affects the parasite transcriptome. Overall design: HFFs were left untreated or pre-treated with IFNg for 24 hours prior to infection, then subsequently left uninfected or infected with wildtype (RH?Ku80), GRA57 knockout (RH?GRA57) or GRA57 complemented (RH?GRA57::GRA57-HA) parasites. All conditions were perfomed in triplicate. Total RNA was isolated 24 hours post-infection, then RNA-Seq was performed using NovaSeq. For human and Toxoplasma-aligned transcriptomes we ran differential analysis across strain and IFNg treatment groups using DESeq2.